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General Category >> Killifish Breeding Rearing and General Keeping >> Micro-worm freezing for long term preservation https://aka.org/cgi-bin/yabb2/YaBB.pl?num=1555071236 Message started by James Langan on Apr 12th, 2019 at 7:13am |
Title: Micro-worm freezing for long term preservation Post by James Langan on Apr 12th, 2019 at 7:13am
Has anyone had direct experience in freezing micro-worm cultures for long periods of time. I though it might be a great way to have a backup culture for unexpected culture loss, so never to have a lack of micro-worms in the fish room. I've heard this has been done and is possible.
Please followup post your experiences, Thanks, Jim Langan |
Title: Re: Micro-worm freezing for long term preservation Post by James Langan on May 3rd, 2019 at 2:29pm
I froze as a test a small culture of micro-worms for two (2) weeks in standard refrigerator freezer. After two weeks thawed out culture and culture was sterile.
Culture contained after three days no (0) viable micro-worms after thawing. I will try a deep freeze next in a -10 dgF deep freezer and report. Jim Langan |
Title: Re: Micro-worm freezing for long term preservation Post by Justin Woody on Oct 24th, 2019 at 10:09am
Interesting, Jim have you tried the deep freeze method and seen if it works?
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