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General Category >> Killifish Breeding  Rearing  and General Keeping >> Micro-worm freezing for long term preservation
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Message started by James Langan on Apr 12th, 2019 at 7:13am

Title: Micro-worm freezing for long term preservation
Post by James Langan on Apr 12th, 2019 at 7:13am
Has anyone had direct experience in freezing micro-worm cultures for long periods of time. I though it might be a great way to have a backup culture for unexpected culture loss, so never to have a lack of micro-worms in the fish room.  I've heard this has been done and is possible.

Please followup post your experiences,

Thanks,

Jim Langan

Title: Re: Micro-worm freezing for long term preservation
Post by James Langan on May 3rd, 2019 at 2:29pm
I froze as a test a small culture of micro-worms for two (2) weeks in standard refrigerator freezer.  After two weeks thawed out culture and culture was sterile.

Culture contained after three days no (0) viable micro-worms after thawing. I will try a deep freeze next in a -10 dgF deep freezer and report.

Jim Langan

Title: Re: Micro-worm freezing for long term preservation
Post by Justin Woody on Oct 24th, 2019 at 10:09am
Interesting, Jim have you tried the deep freeze method and seen if it works?

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